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URL: http://antibodyregistry.org/AB_626004
Proper Citation: GeneTex Cat# GTX80751, RRID:AB_626004
Target Antigen: Orphanin FQ antibody
Host Organism: guinea pig
Clonality: polyclonal
Comments: Discontinued; manufacturer recommendations: Immunohistochemistry. Immunohistochemistry 1:500 (Dilutions listed only as a recommendation. Optimal dilution should be determined by investigator.) Immunohistochemistry: Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10 sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10 sucrose in PBS.Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3
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No rating or validation information has been found for Orphanin FQ antibody.
Discontinued
Discontinued at GeneTex
Source: Antibody Registry
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