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Ken-ichi Miyamoto; Tokushima University; Japan Cat# NaPi2 2011, RRID:AB_2722756Copy Citation Copied
URL: http://antibodyregistry.org/AB_2722756
Proper Citation: Ken-ichi Miyamoto; Tokushima University; Japan Cat# NaPi2 2011, RRID:AB_2722756
Target Antigen: NaPi-2
Host Organism: rabbit
Clonality: polyclonal
Comments: "An antibody was raised against a peptide that represented amino acid sequences (Leu-Ala-Leu-Pro-Ala-His-His-Asn Ala-Thr-Arg-Leu) in the C-terminal region of the Na-P; cotransporter (321-331), corresponding to a putative C terminal intracellular domain (8). An N-terminal cystein residue was introduced for conjugation with keyhole limpet hemocyanine (KLH, Sigma, St Louis, MO) using m-male imidobenzoyl-N-hydroxysuccinimide ester (MBS). The conjugates (100ug of peptide) were mixed with Freund's complete adjuvant and injected sc into rabbits 4 times at 2-week intervals. In the affinity purification by use of a gel column (Cellulofine, Seikagaku-Kogyo, Tokyo), the anti serum (10ml) was loaded on the gel coupled with the peptide and the NaPi-2 antibodies were eluted with 0.1M glycine-HCI (pH 2.5), neutralized, dialyzed, and stored at -80°C."
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Source: Antibody Registry
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