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Antibody Name
Recombinant mouse Anti-KIF3B antibody
RRID:AB_2715472 RRID Copied  
PDF Report How to cite
N. Hirokawa - University of Tokyo, Tokyo, Japan Cat# KIF3B, RRID:AB_2715472
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Antibody Information

URL: http://antibodyregistry.org/AB_2715472

Proper Citation: N. Hirokawa - University of Tokyo, Tokyo, Japan Cat# KIF3B, RRID:AB_2715472

Target Antigen: KIF3B

Host Organism: rabbit

Clonality: polyclonal

Comments: Described in Yamazaki et al., J Cell Biol (1995): "To produce an antibody against KIF3B, the recombinant full-length KIF3B protein was used. Purified KIF3B was injected into rabbits four times and the rabbits were bled. The serum was then affinity purified us- ing a KIF3B-bound activated Thiol-Sepharose 4B affinity column (Phar- macia Inc.). Western blotting of the final antibodies was carried out to confirm no cross-reaction with KIF3A. For the motility inhibition assay, purified antibodies were dialyzed against a PEM buffer (0.1 M Pipes, pH 6.8, 1mM EGTA, i mM MgC12)."

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Authentication Plan

dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.