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Antibody Name
MCH Melanin-concentrating hormone
RRID:AB_2616562 RRID Copied  
PDF Report How to cite
Risold PY Cat# 1153, RRID:AB_2616562
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Antibody Information

URL: http://antibodyregistry.org/AB_2616562

Proper Citation: Risold PY Cat# 1153, RRID:AB_2616562

Target Antigen: Synthetic Salmon MCH; full 17 amino acid sequence: DTMRKMVGRVYRPCWEV

Host Organism: rabbit

Clonality: polyclonal

Comments: Antibody prepared in 1987 by Risold and Fellmann, against the synthetic peptide coupled to the bovine serum albumin.Specificity of the antibody has been verified using liquid phase inhibition, dot blot, affinity column. We also verified the co-expression in the same lateral hypothalamic neurons of the peptide by immunohistochemistry and its ARNm by in situ hybridization in several species including rat. Fellmann D, Bugnon C, Risold PY. 1987. Unrelated peptide immunoreactivities coexist in neurons of the rat lateral dorsal hypothalamus: human growth hormone-releasing factor1-37-, salmon melanin-concentrating hormone- and alpha-melanotropin-like substances. Neurosci Lett 74:275–280. Risold PY, Fellmann D, Rivier J, Vale W, Bugnon C. 1992. Immunoreactivities for antisera to three putative neuropeptides of the rat melanin-concentrating hormone precursor are coexpressed in neurons of the rat lateral dorsal hypothalamus. Neurosci Lett 136:145–149.

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Data and Source Information
Authentication Plan

dkNET can assist you in preparing authentication plans for antibodies to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for antibodies is based on methods suggested in "A proposal for validation of antibodies" (Uhlen M et. al., 2016), the guideline published in the Journal of Comparative Neurology (Saper C, 2005)(2), and the example " Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating antibodies, check our Authentication Reports services.