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Cell Line Name
SEES3-1V human RFX2, clone1
RRID:CVCL_A5V3 RRID Copied  
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RRID:CVCL_A5V3
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Cell Line Information

URL: https://web.expasy.org/cellosaurus/CVCL_A5V3

Proper Citation: RRID:CVCL_A5V3

Description: Cell line SEES3-1V human RFX2, clone1 is a Embryonic stem cell with a species of origin Homo sapiens (Human)

Sex: Male

Defining Citation: PMID:32433964

Comments: Characteristics: Transfected with a pG-flox-CAGrtTA3G-IRES-Hyg-pA construct that contains a reverse tetracycline-controlled transactivator (rtTA), a fusion between a mutated version of E.coli TetR and the activating domain of HSV-1 VP16., Characteristics: Transposed with a PB-PuroR-Ins-tetO2-ORF-IRES-bgeo-PB where PB is a piggyBac sequence, Ins is a chicken HS4 insulator, ORF is the transfected human gene and bgeo is a neo-LacZ fusion protein., Characteristics: When doxycycline is added the transfected human gene (ORF) and lacZ are expressed.

Category: Embryonic stem cell

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Data and Source Information

Source: Cellosaurus (RRID:SCR_013869)
Description: Database of all cell lines used in biomedical research which include immortalized cell lines, naturally immortal cell lines (stem cells), widely used and distributed finite life cell lines, vertebrate cell lines (majority being human, mouse, and rat), and invertebrate (insects and ticks) cell lines, as well as cell line synonyms. Each cell line is provided with the following information: the recommended name (the name which appears in the original publication), a list of synonyms, a unique accession number, comments on a number of topics including misspellings and gene transfection, information on the tissue/organ origin with the UBERON code, the NCI Thesaurus or Orphanet ORDO code for the disease(s) the individual suffered from (for cancer and human genetic disease lines only), the species of origin, the parent cell line, cross-references of sister cell lines, the sex of the individual, the category in which the cell line belongs (Adult stem cell; Cancer cell line; Embryonic stem cell; Factor-dependent cell line; Finite cell line; Hybrid cell line; Hybridoma; Induced pluripotent stem cell; Spontaneously immortalized cell line; Stromal cell line; Telomerase immortalized cell line; Transformed cell line; Undefined cell line type), web links, publication references, and/or cross-references to cell line catalogs/collections, ontologies, cell lines databases/resources, and to databases that list cell lines as samples.
URL: https://www.cellosaurus.org/

Authentication Plan

dkNET can assist you in preparing authentication plans for cell lines to comply with the NIH Submission Policy. The information can be used while planning your experiments or submitting grant applications. The authentication plan for the cell lines is based on the International Cell Line Authentication Committee (ICLAC)'s "Cell Line Checklist for Manuscripts and Grant Applications" and the example "Authentication of of Key Biological and/or Chemical Resources "(Bandrowski A). For best practices for authenticating cell lines, check our Authentication Reports services.