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URL: https://web.expasy.org/cellosaurus/CVCL_E8FJ
Proper Citation: RRID:CVCL_E8FJ
Description: Cell line HCC827(gFGFR1) is a Cancer cell line with a species of origin Homo sapiens (Human)
Sex: Female
Disease: Lung adenocarcinoma
Defining Citation: PMID:30562682
Comments: Characteristics: FGFR1 gene expression was genetically up-regulated. First transduced with a CRISPR-based Synergistic Activation Mediator (SAM) system composed of two lentiviral vectors dCAS-VP64_Blast (EF1a-dCas9-VP64-Bsd-WPE) and MS2-P65-HSF1-Hygro (EF1a-MS2(N55K)-SV40(NLS)-RELA-HSF1-hygR-WPRE) and then a PiggyBac transposon containing three unique SAM compatible gRNAs spanning the promoter region of FGFR1 (gFGFR1) and a puroR-IRES-AsRed-CMV construct (PubMed=30562682)., Population: Caucasian.
Category: Cancer cell line
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Source: Cellosaurus
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