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NeuroMab Tutorial


For up to date issues or problems with this database please see the wiki.
For more information, please visit NeuroMab.


NeuroMab provides low cost, high quality mouse monoclonal antibodies to the neuroscience community. These are generated by neuroscientists; fully validated for biochemical and immunohistochemical applications in mammalian brain.

Mouse monoclonal antibodies are generated from hybridoma cells grown either in vitro or in vivo. The different forms are as follows:

TC supernatant: conditioned bovine serum-containing media harvested from hybridomas cultured in vitro. Typically contains 10-50 micrograms/ml mouse monoclonal IgG plus bovine IgG and other bovine serum components.

From NIF, we can search for any protein and if an antibody is available for that protein, then a record for the antibody shows up.


Neuromab tutorial 1


Autocomplete is a feature that was developed to help search through the vocabulary that is present in NIF and if you select from the autocomplete options then it is most likely that the terms are both spelled correctly and NIF knows about their existence. Here we have chosen to search for the Kv1.1 protein and the results from the database tab under the category "antibodies" are below:


Neuromab tutorial 2


From these results there are two possible links. The accession number column links to the NCBI database where one can explore information about the particular target. The Data_Sheet_ID links to the data sheets for each antibody. See the following results below:


Neuromab tutorial 3


Here are the results for the material data sheet for the Kv1.1 antibody, where there is a wealth of information from where the antibody was obtained to immunoblots. The information at the top can be used to purchase the antibody directly from NeuroMab.


Neuromab tutorial 4


For more information, please visit NeuroMab.

Last updated: Friday, 30-Jul-2010 22:03:09 PDT

For general information, contact us at support@neuinfo.org


Principal Investigators:
Maryann Martone
maryann@ncmir.ucsd.edu

Amarnath Gupta
gupta@sdsc.edu


Jeffrey S. Grethe
jgrethe@ncmir.ucsd.edu

Project Manager:
Ashraf Memon
amemon@sdsc.edu
Curation:
Anita Bandrowski
abandrowski@ucsd.edu
External Relations/Web Support:
Lee G. Hornbrook
lee@ncmir.ucsd.edu